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. 2026 May 20;16:15717. doi: 10.1038/s41598-026-52002-y

Table 3.

Estimation of CAF and CUR in raw materials.

Method I Method II
CAF at (443 nm) CAF at (323 nm) CUR at (437 nm)
Parameter Concentration %founda Concentration %founda Concentration %founda
Taken (ng/mL) Found (ng/mL) Taken (ng/mL) Found (ng/mL) Taken (ng/mL) Found (ng/mL)
30.0 29.211 97.37 50.0 49.869 99.74 20.0 19.638 98.19
50.0 49.225 98.45 200.0 197.891 98.95 50.0 49.029 98.06
100.0 98.514 98.51 500.0 498.163 99.63 70.0 70.307 100.44
200.0 198.845 99.42 700.0 709.801 101.40 100.0 99.039 99.04
400.0 406.036 101.51 1000.0 991.252 99.13 120.0 122.159 101.80
600.0 605.586 100.93 1500.0 1506.88 100.46 150.0 152.868 101.91
800.0 793.310 99.16 2000.0 1995.93 99.80 200.0 197.189 98.59
X̅ ± SD 99.34 ± 1.45 99.87 ± 0.83 99.72 ± 1.66
%RSD 1.46 0.83 1.66
Comparison method [4] (n = 3) Comparison method [4] (n = 3) Comparison method [18] (n = 3)
X̅ ± SD 100.08 ± 1.16 100.08 ± 1.16 99.85 ± 0.80
t b 0.86 (2.31) 0.28 (2.31) 0.17 (2.31)
Fb 1.56 (5.14) 1.95 (5.14) 4.31 (5.14)

aAverage of three replicate determinations.

b The theoretical t and F values (P = 0.05) are between parentheses44.