Table 2. TP53 hotspot mutations identified in tumor tissues of patients with high-grade serous ovarian cancer for ctDNA detection.

Subject number	Histology	Gene	Category	Exon	Mutation	Amino acid change	Effect
P-0002	HGSOC	TP53	Tumor suppressor	8	c.796G>C	p.G266R	Missense
P-0005	HGSOC	TP53	Tumor suppressor	5	c.524G>A	p.R175H	Missense
P-0018	HGSOC	TP53	Tumor suppressor	5	c.413C>T	p.A138V	Missense
P-0019	HGSOC	TP53	Tumor suppressor	4	c.318C>A	p.S106R	Missense
P-0026	HGSOC	TP53	Tumor suppressor	8	c.824G>A	p.C275Y	Missense
P-0029	HGSOC	TP53	Tumor suppressor	8	c.856G>A	p.E286K	Nonsense
P-0032	HGSOC	TP53	Tumor suppressor	8	c.817C>T	p.R273C	Missense
P-0039	HGSOC	TP53	Tumor suppressor	6	c.637G>T	p.R213L	Missense
P-0043	HGSOC	TP53	Tumor suppressor	5	c.488A>G	p.Y163C	Missense
P-0046	HGSOC	TP53	Tumor suppressor	8	c.844C>T	p.R282W	Missense
P-0047	HGSOC	TP53	Tumor suppressor	6	c.659A>G	p.Y220C	Missense
P-0051	HGSOC	TP53	Tumor suppressor	8	c.844C>G	p.R282G	Missense
P-0056	HGSOC	TP53	Tumor suppressor	6	c.584T>C	p.I195T	Missense
P-0064	HGSOC	TP53	Tumor suppressor	7	c.742C>T	p.R248W	Missense
P-0065	HGSOC	TP53	Tumor suppressor	10	c.1084A>T	p.S362C	Missense
P-0074	HGSOC	TP53	Tumor suppressor	4	c.154C>T	p.Q52*	Nonsense
P-0076	HGSOC	TP53	Tumor suppressor	6	c.610G>T	p.E204*	Nonsense
P-0077	HGSOC	TP53	Tumor suppressor	7	c.722C>T	p.S241F	Missense
P-0079	HGSOC	TP53	Tumor suppressor	7	c.742C>T	p.R248W	Missense
P-0080	HGSOC	TP53	Tumor suppressor	8	c.817C>T	p.R273C	Missense
P-0083	HGSOC	TP53	Tumor suppressor	4	c.326T>C	p.F109S	Missense
P-0084	HGSOC	TP53	Tumor suppressor	6	c.568C>A	p.P190T	Missense
P-0085	HGSOC	TP53	Tumor suppressor	7	c.713G>A	p.C238Y	Missense
P-0089	HGSOC	TP53	Tumor suppressor	8	c.818G>A	p.R273H	Missense
P-0102	HGSOC	TP53	Tumor suppressor	6	c.672G>T	p.E224D	Missense

ctDNA, circulating tumor DNA; HGSOC, high grade serous ovarian cancer.