Figure 1.

Signal tranducer and activator of transcription 1 phosphorylation at Ser727 in the absence of protein kinase C-δ. Macrophages derived from wild-type or protein kinase C-δ (Pkc-δ)-deficient mice were treated with interferon (IFN)-β, IFN-γ or lipopolysaccharide (Lps) for 60 min. Stat1 phosphorylation was analysed by western blotting with an antiserum that specifically recognizes the Ser727-phosphorylated molecule (Stat1-pSer727). For normalization, the blot was reprobed with a polyclonal antiserum to the Stat1 carboxyl terminus (Stat1C).