Figure 5.

Antiviral activity. Fibroblasts that express Stat1-α, Stat1-β, Stat1-S727A or no form of Stat1 were treated with serial dilutions of interferon-β (IFN-β) for 14 h. The cells were then infected with vesicular stomatitis virus (VSV) at an MOI (multiplicity of infection) of 0.1. After 24 h, remaining viable cells were stained with crystal violet and staining intensity was determined by absorbance measurements at 620 nm.