Figure 3.

Cbl does not affect the stability of CrkL. (A) Thymocytes were stimulated for various durations and cell lysates were blotted with antibodies as indicated. (B) Thymocytes were pulse-labelled with [³⁵S]methioine for 1 h and cells were chased for various durations. Cell lysates were immunoprecipitated with anti-CrkL or anti-C3G, samples were separated by SDS–polyacrylamide gel electrophoresis, and autoradiography was carried out. The data were quantified and normalized. The original intensity was considered to be the basal level (1.0) for each row. The relative intensity is labelled below each panel.