Figure 7.

Phosphorylation of p130Cas and paxillin was decreased in CFKO mice. Osmotic minipumps containing Ang II were implanted into control and CFKO mice as described in Methods. At the indicated times, mice were euthanized and hearts were dissected. Lysates from the dissected hearts were immunoprecipitated with anti-p130Cas antibody followed by Western blotting with anti-PY20 or with anti-p130Cas or analyzed directly by Western blotting with anti–pY118-paxillin (p-paxillin) or anti-paxillin, as indicated. All blots are representative of at least 3 independent experiments.