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. 2005 Dec 22;116(1):59–69. doi: 10.1172/JCI25074

Figure 5.

Figure 5

CD68S vectors drive the overexpression of MMP-9 in macrophages and atherosclerotic lesions in apoE–/– mice. MMP-9 expression in thioglycollate-elicited peritoneal macrophages (Thio) and aortic arch lysates from apoE–/– mice transplanted with HSCs transduced with CD68S–MMP-9 or CD68S–MMP-9 G100L retroviruses was analyzed by gelatin zymography as described in the Figure 2A legend. Immunoblotting with an antibody against macrosialin (α-mCD68) served as loading control for the isolated macrophages and a measure of macrophage content of lesions in the aortic arch. The proforms and active forms of MMP-9 and MMP-2 are highlighted.