Figure 5.

Islet growth and β cell size. (A) Representative pancreas sections from 9-week-old nonfasted male mice of each group were immunostained with antibodies against insulin (green) or glucagon (red). Magnification, ×100. (B) Plasma insulin levels (average ± SEM) were measured in 8-week-old male mice (at least 8 per genotype) after a 16-hour overnight fast. Morphometric analysis was performed on 20 immunostained pancreatic sections from WT, LKO, Irs1^–/–, and LKO::Irs1^–/– mice. (C) The β cell area is the mean ± SEM percentage of the total pancreatic area surveyed. (D) The β/α cell ratio represents the total β cell number divided by the α cell number in at least 5 pancreatic sections surveyed. (E) Islet size is expressed as the average ± SEM in square micrometers.