Skip to main content
. 2005 Dec 22;116(1):125–136. doi: 10.1172/JCI26040

Figure 1. An siRNA-mediated screen identifies Nrip1 (RIP140) as a negative regulator of deoxyglucose uptake in 3T3-L1 adipocytes.

Figure 1

(A) Four days after the induction of differentiation, 3T3-L1 adipocytes were transfected with smart pools of siRNA against a panel of genes (GenBank accession numbers are provided in Supplemental Table 1) selected as highly expressed in muscle and 3T3-L1 adipocytes compared with 3T3-L1 fibroblasts. The effect of each knockdown on glucose uptake was determined using a 2-deoxyglucose uptake assay. Arrow indicates insulin-stimulated 2-deoxyglucose uptake in RIP140-knockdown cells. Shown is the average of 2 independent experiments. (B) siRNA-mediated depletion of RIP140 mRNA was confirmed by real-time RT-PCR. The graph represents the mean ± SEM of 3 independent experiments. Scr, scrambled; Q-RT-PCR, quantitative RT-PCR. *P < 0.05 compared with scrambled.