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. 2005 Dec 22;116(1):125–136. doi: 10.1172/JCI26040

Figure 2. RIP140 depletion enhances GLUT4 expression and hexose uptake in both day-4 and day-8 3T3-L1 adipocytes.

Figure 2

Four (day 4) or eight (day 8) days after the induction of differentiation, 3T3-L1 adipocytes were transfected with scrambled or RIP140 siRNA. Three days later, cell lysates were used for SDS-PAGE to assess GLUT4, GLUT1, and actin expression levels. Intact cells were used to examine the effect of RIP140 depletion on 2-deoxyglucose uptake. The graph shows the mean ± SEM of 5 independent experiments. *P < 0.05 compared with scrambled, by Student’s t test.