Figure 7. RIP140 depletion enhances glycolysis and TCA cycling, but not triglyceride synthesis or de novo free fatty acid synthesis.

Eight days after the induction of differentiation, 3T3-L1 adipocytes were transfected with scrambled or RIP140 siRNA. After 72 hours, cells were starved and glucose metabolism (with continued starvation [basal] or 1 μM insulin stimulation) was measured by [6-¹⁴C]-glucose conversion into carbon dioxide (CO₂), triglycerides, and fatty acids as described in Methods. Graphs show the mean ± SEM of 3–4 independent experiments. *P < 0.05 compared with scrambled, by Student’s t test.