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. 2026 May 11;17:1802250. doi: 10.3389/fimmu.2026.1802250

Figure 3.

Panel A shows a UMAP plot of clustered cell types with color-coded groups. Panel B displays a dot plot of multiple gene expression levels across cell types. Panels C, E, and G are violin plots of C5ar1, Il1b, and Sema3a gene expression by cell type, respectively. Panels D, F, and H present group comparisons of these genes between Sham and PAB, highlighting statistical significance. Panel I contains fluorescent microscopy images comparing C5aR1, CD68, and CD8 staining with DAPI nuclear stain in PAB and Sham groups, including a zoomed-in view.

Single-cell RNA sequencing of P7 lungs. (A) Cell cluster annotation. (B) Violin plot displaying. (C) C5aR1 expression distribution. (D) Expression of C5aR1 in PAB and Sham. (E) IL-1β expression distribution. (F) Expression of IL-1β in PAB and Sham. (G) Sema3a expression distribution. (H) Expression of Sema3a in PAB and Sham. (I) Subcellular localization of C5aR1 in macrophages. *: P≤0.05; ***: P≤0.001; ****: P≤0.0001.