Fig. 2.

Residue conservation and functional annotation of sheep MC4R predicted by ConSurf. Amino acid residues are colored according to their evolutionary conservation scores (1–9), where highly conserved residues are shown in dark colors and variable residues in lighter colors. Residues predicted to be functionally important (highly conserved and exposed) and structurally important (highly conserved and buried) are indicated. The positions of analyzed nsSNPs are highlighted to show their distribution across conserved and variable regions.