Figure 2.

Aubergine does not impact the presence of piRNAs in Drosophila intestinal stem/progenitor cells

(A) Schematic of sorted ISCs/EBs (green dots) used for small RNA sequencing.

(B–C′) Size distribution (top) of all transposon-mapping reads and uridine and adenine frequencies of piRNA size (>22 nt) (bottom) transposon-mapping reads in oxidized libraries from sorted ISCs/EBs.

(D and E) Quantification of TE antisense (D) and sense (E) piRNAs abundance as in (B–C’). Two-way ANOVA followed by Sidak’s multiple comparisons tests were applied. n = 3 biological replicates.

(F) Representation of piRNA clusters expression in sorted ISCs/EBs as in (B–C′), compared to ovaries.

Results are presented relative to control ovaries. a.u., arbitrary units. TE, transposable element; AS, antisense; S, sense. ns, not significant. Data are represented as mean ± SD.