Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1981 Oct;45(4):343–351.

Experimental aflatoxicosis in swine: morphological and clinical pathological results.

D M Miller, B P Stuart, W A Crowell
PMCID: PMC1320161  PMID: 7337866

Abstract

The morphological changes in livers of 30 feeder pigs fed diets containing corn contaminated by aflatoxins (0.0 microgram aflatoxins/g feed, 0.4 microgram aflatoxin/g feed, and 0.8 microgram aflatoxin/g feed) were compared with changes in hematology, liver specific serum enzymes, serum proteins, and lymphocyte stimulation indices. Histologically, the livers were classified into five groups. Pigs fed the 0.8 microgram/g diets had the most severe histological lesions of karyomegaly, bile ductule proliferation and hepatocellular degeneration plus elevated gamma-glutamyl transpeptidase, aspartate aminotransferase, and alkaline phosphatase. This group also had significantly lower total protein and albumin values compared to the control pigs. Variation in the severity of the histological lesions was seen in pigs fed 0.4 microgram/g diets as well as variation in lymphocyte indices, liver specific serum enzymes, and electrophoretic results in the affected pigs in that group.

Full text

PDF
346

Images in this article

345Fig. 1.
on p.345
345Fig. 2.
on p.345
346Fig. 3.
on p.346
346Fig. 4.
on p.346
347Fig. 5.
on p.347

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURNSIDE J. E., SIPPEL W. L., FORGACS J., CARLL W. T., ATWOOD M. B., DOLL E. R. A disease of swine and cattle caused by eating moldy corn. II. Experimental production with pure cultures of molds. Am J Vet Res. 1957 Oct;18(69):817–824. [PubMed] [Google Scholar]
  2. Burke M. D. Hepatic function testing. Postgrad Med. 1978 Sep;64(3):177-82, 185. doi: 10.1080/00325481.1978.11714929. [DOI] [PubMed] [Google Scholar]
  3. Butler W. H. Early hepatic parenchymal changes induced in the rat by aflatoxin B1. Am J Pathol. 1966 Jul;49(1):113–128. [PMC free article] [PubMed] [Google Scholar]
  4. Carnaghan R. B., Hebert C. N., Patterson D. S., Sweasey D. Comparative biological and biochemical studies in hybrid chicks. 2. Susceptibility to aflatoxin and effects on serum protein constituents. Br Poult Sci. 1967 Oct;8(4):279–284. doi: 10.1080/00071666708415680. [DOI] [PubMed] [Google Scholar]
  5. Clampitt R. B., Hart R. J. The tissue activities of some diagnostic enzymes in ten mammalian species. J Comp Pathol. 1978 Oct;88(4):607–621. doi: 10.1016/0021-9975(78)90014-2. [DOI] [PubMed] [Google Scholar]
  6. Clifford J. I., Rees K. R. The action of aflatoxin B1 on the rat liver. Biochem J. 1967 Jan;102(1):65–75. doi: 10.1042/bj1020065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cysewski S. J., Wood R. L., Pier A. C., Baetz A. L. Effects of aflatoxin on the development of acquired immunity to swine erysipelas. Am J Vet Res. 1978 Mar;39(3):445–448. [PubMed] [Google Scholar]
  8. Gagné W. E., Dungworth D. L., Moulton J. E. Pathologic effects of aflatoxin in pigs. Pathol Vet. 1968;5(4):370–384. doi: 10.1177/030098586800500407. [DOI] [PubMed] [Google Scholar]
  9. Gumbmann M. R., Williams S. N. Biochemical effects of aflatoxin in pigs. Toxicol Appl Pharmacol. 1969 Sep;15(2):393–404. doi: 10.1016/0041-008x(69)90037-4. [DOI] [PubMed] [Google Scholar]
  10. Hartzman R. J., Bach M. L., Bach F. H., Thurman G. B., Sell K. W. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor. Cell Immunol. 1972 Jun;4(2):182–186. doi: 10.1016/0008-8749(72)90018-4. [DOI] [PubMed] [Google Scholar]
  11. Michael G. Y., Thaxton P., Hamilton P. B. Impairment of the reticuloendothelial system of chickens during aflatoxicosis. Poult Sci. 1973 May;52(3):1206–1207. doi: 10.3382/ps.0521206. [DOI] [PubMed] [Google Scholar]
  12. Naftalin L., Child V. J., Morley D. A., Smith D. A. Observations on the site of origin of serum gamma-glutamyltranspeptidase. Clin Chim Acta. 1969 Nov;26(2):297–300. doi: 10.1016/0009-8981(69)90382-9. [DOI] [PubMed] [Google Scholar]
  13. Newberne P. M., Butler W. H. Acute and chronic effects of aflatoxin on the liver of domestic and laboratory animals: a review. Cancer Res. 1969 Jan;29(1):236–250. [PubMed] [Google Scholar]
  14. Newberne P. M. Chronic aflatoxicosis. J Am Vet Med Assoc. 1973 Dec 1;163(11):1262–1267. [PubMed] [Google Scholar]
  15. Paul P. S., Johnson D. W., Mirocha C. J., Soper F. F., Thoen C. O., Muscoplat C. C., Weber A. F. In vitro stimulation of bovine peripheral blood lymphocytes: suppression of phytomitogen and specific antigen lymphocyte responses by aflatoxin. Am J Vet Res. 1977 Dec;38(12):2033–2035. [PubMed] [Google Scholar]
  16. Pier A. C. Effects of aflatoxin on immunity. J Am Vet Med Assoc. 1973 Dec 1;163(11):1268–1269. [PubMed] [Google Scholar]
  17. Pier A. C., Fichtner R. E., Cysewski S. J. Effects of aflatoxin on the cellular immune system. Ann Nutr Aliment. 1977;31(4-6):781–788. [PubMed] [Google Scholar]
  18. Richard J. L., Thurston J. R. Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages. Appl Microbiol. 1975 Jul;30(1):44–47. doi: 10.1128/am.30.1.44-47.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SUNDERMAN F. W., Jr STUDIES ON THE SERUM PROTEINS. VI. RECENT ADVANCES IN CLINICAL INTERPRETATION OF ELECTROPHORETIC FRACTIONATIONS. Am J Clin Pathol. 1964 Jul;42:1–21. doi: 10.1093/ajcp/42.1.1. [DOI] [PubMed] [Google Scholar]
  20. Savel H., Forsyth B., Schaeffer W., Cardella T. Effect of aflatoxin B1 upon phytohemagglutinin-transformed human lymphocytes. Proc Soc Exp Biol Med. 1970 Sep;134(4):1112–1115. doi: 10.3181/00379727-134-34954. [DOI] [PubMed] [Google Scholar]
  21. Sisk D. B., Carlton W. W., Curtin T. M. Experimental aflatoxicosis in young swine. Am J Vet Res. 1968 Aug;29(8):1591–1602. [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES