Table 1.
List of primers used for PCR.
| Primer name (5′ → 3′) | Forward | Reverse |
|---|---|---|
| pUC19_linearisation | AGCTGTTTCCTGTGTGAAATTGTTATCCG | TTAAGCCAGCCCCGACACCC |
| pUC19_sequencing | TGTAAGCGGATGCCGGGAGCAGACAAGC | ACACTTTATGCTTCCGGCTCG |
| Fragment_12_linearisation | TCGGGGCTGGCTTAACGGGCTTTCCTTGATGGGCG | ACACAGGAAACAGCTATCCTGGCGCTTGTATTCCCCC |
| pUC19-12_linearisation | GGCTGGTCAAACGGGTGACGGTGACATACCGCCACG | GCTACGAAGTGTTCCTCGGGCTTTCCTTGATGGGCG |
| pBADΔ12_linearisation | GGGAATACAAGCGCCAGGATCTGGTTTACGAGAAAGAGGA | CGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAG |
| Fragment_1_linearisation | TCGGGGCTGGCTTAAGCTAGCGAATTCGAGCTCGGTACCC | ACACAGGAAACAGCTACCCATTTTGGTTCCAGCATCTGGTC |
| pUC19-1_linearisation | TACCCGTTTTTTTGGGCTAGCGAATTCGAGCTCGGTACCC | GCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTC |
| pBADΔ1_linearisation | ATGCTGGAACCAAAATGGGTTATCTCAATGGGTGCCTGTG | CCGAGCTCGAATTCGCTAGCCCAAAAAAACGGGTATGGAG |
| pUC19-12_E216Q | GTTTGACCAGCCGCAAGCCGAG | CGGCTGGTCAAACGGGTGACG |
| pUC19-12_E218Q | GGAAGCCCAGCAGGAACTGG | GGCTTCCGGCTGGTCAAACGG |
| pUC19-12_E218R | GGAAGCCCGGCAGGAACTGG | GGCTTCCGGCTGGTCAAACGG |
| pUC19-12_E241Q | GTTCTTCGTGGGTCAGTACATCGGGATTG | ACCCACGAAGAACAGACCGAACTTC |
| pUC19-12_E241A | GTTCTTCGTGGGTGCGTACATCGGGATTG | ACCCACGAAGAACAGACCGAACTTC |
| pUC19-13_I63M | GTCTACGCGGGTGCCATGATGGTGCTGTTCGTGTTCGTGGTGATG | GGCACCCGCGTAGACGATAATTTCC |
| pUC19-13_I63A | GTCTACGCGGGTGCCGCTATGGTGCTGTTCGTGTTCGTGGTGATG | GGCACCCGCGTAGACGATAATTTCC |
| pUC19-1_D79A | TTCTTCGTTATCTTCGCCGTTGAAGCGCTG | GAAGATAACGAAGAACATGGCCACCAGATAAAAC |
| pUC19-1_E51A | AAAAACGTGCCGTTTGCATCCGGTATCGACTCG | AAACGGCACGTTTTTCGACCTCGCG |