. 2026 May 20;7(7):1359–1371. doi: 10.1039/d6cb00090h

Table 2. Kinetic parameters for the hydrolysis of 3PET by wild-type (WT) LCC and its variants in 50 mM citrate, 100 mM phosphate (pH 8.0) at 45 °C. The k_cat, K_M, and n-values were determined with the Hill equation (Fig. S12).

Enzyme	k _cat (s⁻¹)	K _M (µM)	k _cat/K_M (s⁻¹ mM⁻¹)	n-value
WT	4.04 ± 0.31	114 ± 12	35.6 ± 1.4	1.60 ± 0.09
V212T	4.77 ± 0.13	127 ± 14	36.5 ± 1.3	1.58 ± 0.12
L102F–V212T	6.73 ± 0.14	148.0 ± 2.1	45.4 ± 0.6	1.530 ± 0.066

Table 2. Kinetic parameters for the hydrolysis of 3PET by wild-type (WT) LCC and its variants in 50 mM citrate, 100 mM phosphate (pH 8.0) at 45 °C. The kcat, KM, and n-values were determined with the Hill equation (Fig. S12).