AF	Anti-FLAG affinity chromatography
CDD	CDKL5 Deficiency Disorder
CV	Column volume
E.coli	Escherichia coli
ELISA	Enzyme-Linked Immunosorbent Assay
ERT	Enzyme replacement therapy
eGFP	Enhanced green fluorescent protein
GG	Gluconate–Glutamate medium
GGM	GG medium supplemented with maltose
hCDKL5	Human cyclin-dependent kinase-like 5
HRP	Horseradish peroxidase
IDR	Intrinsically disordered region
IMAC	Immobilised metal affinity chromatography
LC–MS/MS	Liquid chromatography–tandem mass spectrometry
LOD	Limit Of Detection
LOQ	Limit Of Quantification
NaPi	Sodium phosphate buffer
OD600nm	Optical density at 600 nm
PBST	Phosphate-buffered saline with Tween 20
PBS	Phosphate-buffered saline
PCR	Polymerase Chain Reaction
PhTAC125	Pseudoalteromonas Haloplanktis TAC125
QbD	Quality by Design
SDS-PAGE	Sodium dodecyl sulphate–polyacrylamide gel electrophoresis
SUMO	Small ubiquitin-like modifier
TMB	3,3′,5,5′-tetramethylbenzidine
TS	Twin-Strep tag
TYP	Tryptone–yeast extract–peptone medium