Figure 2.
Amino acid diversity and rearrangement of AT-hook motifs in TashAT2/3 alleles (A) Pattern of AT-hook motifs predicted from allelic DNA sequences of TashAT2 and TashAT3. The regions containing the TashAT locus were extracted from 8 publicly available genomes (ENA project ID: PRJEB65114) previously aligned to the C9 Ankara reference strain using BEDtools. The number and positions of AT-hook motifs were identified using SMART and the core (GRP) motif for each putative site validated manually. Relative positions of each AT-hook domain are denoted Orange. (B) The TashAT2 sequences were compared using JCoDA to identify sites of positive selection of amino acid substitution. The relative positions of sites with a probability of (dN/dS > 1) greater than 0.9 (white) were overlaid on a composite cartoon of the TashAT2 alleles.