Table 2.
The primer sequences, amplicon length, melting temperature and GenBank accession number of the studied genes (9).
| Gene name | Gene symbol | Primers sequence | GeneBank accession number | Amplicon length [bp] | Melting temperature [°C] |
|---|---|---|---|---|---|
| cathelicidin 4 (indolicidin) | CATHL4 | ACCCATCCAATGACCAGTTTGACC TTCACTGTCCAGAAGCCCGAATCT | X67340.1 | 177 | 60 |
| cathelicidin 5 (bovine myeloid antimicrobial peptide 28) | CATHL5 (BMAP28) | TCGGGAGTAACTTCGACATCACCT GGCCCACAATTCACCCAATTCTGA | X97609.1 | 141 | 60 |
| cathelicidin 6 (bovine myeloid antimicrobial peptide 27) | CATHL6 (BMAP27) | ATGGGCTGGTGAAGCAATGTGTAG TGGAGTAGCGGAATGACTGGAGAA | X97608.1 | 163 | 60 |
| β-defensin1 (enteric β-defensin) | DEFB1 (EBD) | ATCCTCTAAGCTGCCGTCT AGCATTTTACTGAGGGCGT | NM_175703.3 | 102 | 58 |
| β-defensin4 (bovine neutrophil β-defensin 4) | DEFB4 (BNBD4) | CGTTCTTGTGCCGTGTAG AAATTTTAGACGGTGTGTTG | NM_174775 | 149 | 58 |
| β-defensin5 (bovine neutrophil β-defensin 5) | DEFB5 (BNBD5) | TCCTCGTGCTCCTCTTCCTA CATATTCCAACGGCAGCTTT | NM_001130761 | 143 | 58 |
| β-defensin10 (bovine neutrophil β-defensin 10) | DEFB10 (BNBD10) | AGTTATCTAAGCTGCTGGG CGCTCTGTCAAAGGGTC | NM_001115084 | 173 | 58 |
| tracheal antimicrobial peptide | TAP | GCGCTCCTCTTCCTGGTCCTG GCACGTTCTGACTGGGCATTGA | NM_174776 | 216 | 57 |
| lingual antimicrobial peptide | LAP | GAAATTCTCAAAGCTGCCGTA TCCTCCTGCAGCATTTTACTT | NM_203435 | 194 | 58 |