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. Author manuscript; available in PMC: 2026 May 30.
Published before final editing as: Cell Rep. 2026 Apr 10;45(4):117231. doi: 10.1016/j.celrep.2026.117231

Figure 2. Cryo-EM structure of the DDM-solubilized, crosslinked FtsH·HflK/CSS complex.

Figure 2.

(A) 2D class averages of the HflK/CSS (HflK/CSS) variant under non-reducing conditions show the closed conformation of the assembly.

(B) Under reducing conditions, HflK/CSS adopts an open, nautilus-like conformation.

(C) Density map and cartoon models of HflK (blue) and HflC (cyan) viewed from the side and top, highlighting the closed HflK/C chamber.

(D) Left: close-up view of the overlay between the atomic model and density map of the hat region of HflK (blue) and HflC (cyan), showing exemplary side-chain density. Right: an exemplary density for the disulfide bond between HflK and HflC.