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. Author manuscript; available in PMC: 2026 May 30.
Published before final editing as: Cell Rep. 2026 Apr 10;45(4):117231. doi: 10.1016/j.celrep.2026.117231

Figure 6. Cryo-EM maps of the native HflK/C complex from cells treated with tobramycin.

Figure 6.

The maps are color coded using an atomic model: HflK in blue, HflC in cyan, and FtsH in pink.

(A and B) Cryo-EM reconstructions of DDM-solubilized HflK/C complexes showing a top view and a sliced top view, respectively. Two openings are observed near the positions where FtsH hexamers are embedded within the HflK/C assembly.

(C) Previously determined cryo-EM map of the nautilus-like FtsH·HflK/C complex (EMD-46057).

(D and E) Cryo-EM maps of HflK/C complexes extracted into Carboxy-DIBMA nanodiscs, showing the secondary opening toward the lipid bilayer, as seen from both top (D) and side (E) views. Notably, this map reveals the lipid bilayer surrounding the complex and shows only one strong FtsH periplasmic domain density compared with the DDM-solubilized samples.