Table 1.

Cryo-EM data collection, processing, model building, and validation statistics

Name	Crosslinked HflK/C variant (HflK/C^SS)	HflK/C^WT solubilized in DDM from tobramycin-treated cells	HflK/C^WT extracted in Carboxy-DIBMA from tobramycin-treated cells

PDB ID	9PAW	N/A	N/A
EMDB ID	EMD-71448	EMD-71449	EMD-71447
Microscope	Titan Krios G3	Titan Krios G3i	Titan Krios G3i
Camera	Falcon 4	K3 detector (Gatan)	K3 detector (Gatan)
Magnification	75,000×	130,000×	130,000×
Voltage (kV)	300	300	300
Total electron dose (e⁻/Å²)	52.68 e⁻/Å²	48.02 e⁻/Å²	46.86 e⁻/Å²
Defocus range (μm)	−1.2 to −2.4 μm	−0.5 to −1.75 μm	−1.3 μm
Pixel size (Å)	0.8654	0.654	0.654
Micrographs collected	8,766	21,895	32,694
Final particles	299,919	58,175	24,196
Symmetry	C₁	C₁	C₁
Resolution (Å) FSC 0.143	2.9	9.1	11.5
Unmasked resolution (Å)	4.2	11	16
Sphericity score (out of 1)	0.85	0.90	0.80
All atoms	90,278	N/A	N/A
Protein residues	6,433	N/A	N/A
Ligands	12	N/A	N/A
Map-model CC (mask)	0.76	N/A	N/A
RMSD bond lengths (Å)	0.001	N/A	N/A
RMSD bond angles (deg.)	0.369	N/A	N/A
MolProbity score	1.18	N/A	N/A
Clash score	1.27	N/A	N/A
C-beta outliers (%)	N/A	N/A	N/A
Rotamer outliers (%)	0.00	N/A	N/A
Ramachandran favored (%)	95.18	N/A	N/A
Q-score (global/expected)	0.41/0.53	N/A	N/A