Table 2.

Primers and cycle numbers used in semiquantitative RT-PCR

Gene	Primer sequence (5′-3′)	Product size	PCR cycle number
SREBP-lc	CGGAACCATCTTGGCAACAGT CGCTTCTCAATGGCGTTGT	170	29
SCD	TTCCTACCTGCAAGTTCTACACC CCGAGCTTTGTAAGAGCGGT	202	27
ACC	ATGTCTGGCTTGCACCTAGTA CCCCAAAGCGAGTAACAAATTCT	215	32
MTP	ACAAGCTCACGTACTCCACTG TCCTCCATAGTAAGGCCACATC	261	27
GAPDH	ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA	452	25
FBXW7	AAAGAGTTGTTAGCGGTTCTCG CCACATGGATACCATCAAACTG	236	27