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. 2005 Dec 23;33(22):6961–6971. doi: 10.1093/nar/gki1004

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Overlay of the UV melting curves of the unmodified (ACSL) and ψ₃₂–modified RNA (ψ₃₂-ACSL) hairpins. Each of the hairpins appears to exhibit two melting transitions—the broad lower temperature transitions presumably corresponding to destacking of the loop nucleotide bases. The apparent melting temperatures of the unmodified and ψ₃₂–modified RNA hairpins are estimated to be 77.0°C and 73.5°C, respectively.

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