Table 2. T_m values measured by nanoDSF in triplicate (unless stated otherwise) for UrdA′_WT and UrdA′_R411A in the presence and absence of substrate/product and anions.

Protein	Ligand	Anion	Tm ± SEM (°C)
UrdA′WT	—	None	43.6 ± 0.03 (n = 4)
		10 mM phosphate	46.7 ± 0.18
		100 mM phosphate	48.7 ± 0.17
		100 mM sulfate	46.1 ± 0.30
	Urocanate	None	43.6 ± 0.11 (n = 4)
		10 mM phosphate	46.8 ± 0.08
		100 mM phosphate	49.0 ± 0.12
		100 mM sulfate	45.7 ± 0.13
	Imidazole propionate	None	43.6 ± 0.02 (n = 4)
		10 mM phosphate	46.8 ± 0.05
		100 mM phosphate	49.1 ± 0.18
		100 mM sulfate	45.8 ± 0.03 (n = 2)
UrdA′R411A	—	None	46.4 ± 0.04
		100 mM phosphate	47.6 ± 0.17
		100 mM sulfate	47.2 ± 0.08
	Urocanate	None	46.2 ± 0.12
		100 mM phosphate	47.0 ± 0.03 (n = 2)
		100 mM sulfate	46.7 ± 0.17
	Imidazole propionate	None	46.6 ± 0.23
		100 mM phosphate	47.4 ± 0.36
		100 mM sulfate	47.3 ± 0.28