Figure 2. iNOS is required for normal cytokine production in the presence of K. pneumoniae.

Wild-type and Nos2^−/− primary bone marrow-derived macrophages were infected with KPPR1 for one hour prior to the removal of extracellular bacteria with gentamicin. Supernatant was collected after gentamicin treatment (T0) or four hours post-infection (T4). Macrophage supernatants were evaluated for cytokine abundance using ELISAs for (A) TNFα, (B) MCP-1, (C) IL-6, (D) IL-12p40, and (E) IL-10. *p<0.05 by paired t-test; +p<0.05, +++p<0.001, ++++p<0.0001 by paired t-test comparing cytokine abundance at T4 to T0 for each cell type. For all, p values <0.1 are displayed as text; n=5 trials with symbols representing independent experiments. The top of each bar represents mean values.