Skip to main content
[Preprint]. 2026 May 24:2026.05.21.726867. [Version 1] doi: 10.64898/2026.05.21.726867

Figure 2: TGIF is needed for Golgi structural maintenance.

Figure 2:

(A) Immunofluorescence showing colocalization of mGRASP-mCherry (magenta) and Sec13-GFP (green) in TGIF-Ty:Sec13-GFP parasites treated with ethanol (control) or IAA (knockdown) for 1, 15, and 48 hours. White boxes denote region used to make insets. Scale bars are 5μm. Inset scale bar are 1μm. Representative images from three experimental replicates (N > 35 vacuoles per condition). Images are maximum intensity projections of z-stacked images. (B) Quantification of Golgi area from images in (A). P-values from paired Student’s t-tests are indicated. (C) Transmission electron microscopy images from TGIF-Ty parasites treated with ethanol (control) or IAA (knockdown) for 15 and 36 hours. White boxes denote region used to make insets. The Golgi cisternae are outlined in cyan, and the ER membrane is outlined in yellow in insets. Representative images from two experimental replicates (N > 12 vacuoles per condition). (D) Immunofluorescence showing the ER marker HDEL-GFP (grey) in TGIF-Ty parasites treated with ethanol (control) or IAA (knockdown) for 15 and 24 hours. Scale bars are 5μm. Representative images from two experimental replicates (N > 20 vacuoles per condition). (E) Immunofluorescence of mGRASP-mCherry (magenta, left) and Sec13-GFP (magenta, right) in TGIF-Ty:Sec13-GFP parasites treated with ethanol (control) or IAA (knockdown) for 24 hours. Anti-IMC3 (yellow) used to visualize the periphery of mother and daughter parasites. Scale bars are 5μm. Representative images from three experimental replicates (N > 30 vacuoles per condition).