Fig. 3 |. GGTA1 KO cell lysates induce reduced basophil activation in an indirect basophil activation test.

A, Representative flow cytometry plots of human basophils following stimulation with lysis buffer negative control, beef thyroglobulin positive control, or lysates from control and GGTA1 KO iBSC clones. Basophil activation was measured as the percentage of CD123⁺ cells that were also CD63⁺. B, Quantification of basophil activation induced by lysates from control and GGTA1 KO clones. Each point represents an independent clone; bars show mean ± SD. Groups were compared using an unpaired two-tailed Welch’s t-test (p = 0.0083).