Figure 2: TF combinations drive cell-type specific regulatory programs.

A. Schematic to visualize transcriptional phenotypes from combinatorial TF over-expression. Cells induced with identical TF combinations were combined.

B. For the 1335 TF combinations analyzed, shown is the distribution of the number of TFs in each combination.

C. The heatmap indicates the expression of 3244 variably expressed genes across the 1335 TF combinations.

D. MDE embedding of transcriptomes for 1335 TF combinations. Each dot indicates a TF combination, colored by experimental batch. When a given TF combination is recovered by multiple batches, the size of each dot indicates the fraction of cells derived from the more abundant batch.

E. Across the 1335 TF combinations, shown is the fraction of cells derived from the most abundant batch.

F. MDE embedding, with TF combinations colored by perturbation cluster and annotated with enriched Gene Ontology terms.

G. Heatmap illustrating the enrichment of Gene Ontology terms and pathways across the 51 perturbation clusters.

H. The MA plot indicates the differentially expressed genes in perturbation cluster 1 (teal). Several genes with cardiac roles are indicated.