Table 1. Microarray-based identification of genetic heterogeneity.
| Organism (strain) | Profile* | Implication |
|---|---|---|
| V. cholerae O1 (E8261) | ctxA–, ctxB–, ace+, zot+, rstRCL+ | Incomplete CTXΦ genome (nontoxigenic V. cholerae O1, classical) |
| V. cholerae O1 (75) | rfbN+, nanH–, ctxA+, ctxB+, ace+, zot+, rstRCL+ | Sixth pandemic (1921) toxigenic V. cholerae O1, classical isolate that may predate the acquisition of VPI-2 |
| V. cholerae O1 (3535–02) | rfbN+, nanH–, hlyAET+, rtxA+, hap+, tcpA–, tcpI–, ctxA–, ctxB–, ace–, zot–, rstR– | Nontoxigenic V. cholerae O1, atypical isolate lacking VPI-2 |
| V. cholerae non-O1/non-O139 (3522–00)† | rfbN–, nanH+, wbfR–, wbfO–, otnA+, otnB–, ctxA+, ctxB+, ace+, zot+, rstRCL+, rstRCalc+ | Toxigenic non-O1/non-O139 isolate (serogroup O141) with VPI-2 neuraminidase and multiple (CTXCL and CTXCalc) prophages |
| V. cholerae O139 (K0020)† | dfrA12+, dfrA18+ | Multiple trimethoprim resistance determinants |
| V. parahaemolyticus (F5052)‡ | groEL+, 16S-23S IGS+, gyrB+, pss+, VP1696+, tl+, tdh+, trh+, toxRS/new+ | Nonpandemic group member with the thermolabile hemolysin, thermostable direct hemolysin, thermostable direct hemolysin-related hemolysin, and a chromosome I TTSS marker only§ |
| V. vulnificus (F9546)‡ | wcvH+, vvhA+, vllY+, vvp+, rtxA+, hlyIII+, VV0601+, VV1546+, VV0914+ | Cytolysin-hemolysin, hemolysin, vibriolysin, repeats-in-toxin cytotoxin, and putative hemolysins |
| V. mimicus (2419–94) | tdh+, nanH+, hap+, strA+, strB+, VPA1339+, VPA1346+, VP1696+, vmhA+, vmc+ | Thermostable direct hemolysin, VPI-2 neuraminidase, streptomycin resistance, and putative TTSS genes homologous to escC, yopP, and yscC§ |
*
Partial profile from microarray-based genotyping
‡
The entire hybridization positive profile has been presented. Profile differences can be seen in comparison to the species-matched profiles in Fig. 1
§
V. parahaemolyticus open reading frames VPA1339 (escC) and VPA1346 (yopP) are found on chromosome 2 and VP1696 (yscC) is found on chromosome 1 (5)