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. 2005 Dec 19;102(52):19198–19203. doi: 10.1073/pnas.0505203102

Fig. 3.

Fig. 3.

Expression of BACE activity and protein in mice with age. At 3, 6, or 12 months of age, brain from APP23 (n = 10) and APP23/Ar+/- (n = 13) mice were prepared for enzyme activity and Western blot. The OVX APP mice (n = 14) were OVX at 3 months, and brains were harvested only at 6 or 12 months of age. (A) BACE activity was detected by detection of fluorescent-labeled peptides. (B) BACE protein levels were measured by Western blot by using monoclonal anti-BACE and were normalized according to those of a β-actin control. Bars indicate the mean ratio of BACE/β-actin integrated optical density values (IDV). (Inset) Western blot of 6-month-old mice. (C)Representative immunoblots were immunostained with APPC8, an antibody against the APP C-terminal fragment C99 that results from BACE cleavage of APP, in WT, APP23, and APP23/Ar+/- mice at various ages. *, P < 0.05; **, P < 0.01 (compared with WT mice). ##, P < 0.05 (compared with APP23 mice).