Table 1. Quantitative analysis of ku80-mutant, complemented ku80-mutant, wild-type, and KU80-overexpressing plants.
Plant | Roots producing galls,* % | Tumor morphology† | GUS expression,‡ % of control |
---|---|---|---|
High-concentration inoculum§ | |||
Wild type | 92 ± 3 | Large and green | 100 |
ku80 mutant | 7 ± 1 | Very small, yellow | 95 ± 2 |
Rescued ku80 mutant | 89 ± 4 | Large and green | 96 ± 3 |
Low-concentration inoculum | |||
KU80 overexpression | 88 ± 3 | Very large and green | 52 ± 4 |
Wild type | 42 ± 5 | Large and green | 50 ± 3 |
Numbers represent the percentages of root segments producing galls 5–6 weeks postinoculation (n = 10 plates)
Characterization of tumor morphology, as described (22)
GUS expression levels were calculated as percentages of the control (wild-type plants infected with a high concentration of Agrobacterium inoculum) (n = 10 plates)
High-concentration Agrobacterium inoculum was used for comparison between the wild type, ku80 mutant, and rescued ku80 mutant, whereas low-concentration Agrobacterium inoculum was used for comparison between wild-type and KU80-overexpressing plants