Fig. 2.

Hierarchical dendogram and PCA of the normal cells and visualization of the genetic subtypes in the principal components obtained from PCA of the normal cells. (A) Hierarchical dendogram using Pearson correlation and average linkage of the normal cells, showing that samples cluster according to lineage and that the CD34⁺ samples cosegregate and cluster separated from the mature cells. (B) PCA of normal cell populations, showing that the cells segregate according to their lineages and maturations. In all PCA analyses, the CD71/GPA⁺ cells were removed to better appreciate the structure of the data. (C) The pediatric ALLs, each subtype represented by their centroids (yellow), were projected onto the principal components determined by the normal cells. All PCAs can be viewed in Movies 1–10, which are published as supporting information on the PNAS web site.