Fig. 2.
AEA and BDNF coregulate neurite development of CB1R+ interneurons. (A and A′) Examples of cellular morphologies. (Scale bar: 40 μm.) (B) Resting membrane potentials at 6 DIV (n = 7 cells per condition). (C) BDNF (100 ng/ml) induces neurite extension and branching. In contrast, AEA (100 nM) inhibits neurite formation (n > 20 cells per condition) and suppresses the BDNF-induced neurite growth. AM251 antagonizes the effects of AEA. Significant BDNF–AEA interaction was noted in total length: F(3,47) = 6.696, P = 0.011. All treatments lasted for 6 DIV. Experiments were performed in triplicate. ○, P < 0.05; ○○, P < 0.01 vs. control; *, P < 0.05; **, P < 0.01 vs. 100 ng/ml BDNF; +, P < 0.05 vs. 100 nM AEA; #, P < 0.05 vs. combination.