Table 1. Folding kinetic rate constants for oxidized proteins.
Rate constants,† s-1
|
|||||
---|---|---|---|---|---|
Protein | Burst amplitude* | kN | kVF | kF | kM |
WT | 0.56 | 18.6 | 0.429 | 0.0597 | |
D9N | 0.62 | 3.22 | 0.312 | 0.0496 | |
L107P | 0.56 | 0.735 | 0.178 | 0.0529 | |
A29V | 0.52 | 20.0 | 1.04 | 0.0816 | 0.0240 |
D15N | 0.54 | 18.1 | 0.469 | 0.0536 | |
I41T | 0.55 | 6.316 | 6.38 | 0.122 | 0.0329 |
S95P | 0.53 | 1.13 | 0.215 | 0.0483 |
Given as a fraction of the total amplitude normalized to a value of 1.
kVF, kF, and kM are the rate constants for the very fast, fast, and medium phases, respectively. kN corresponds to the novel phase of positive change in fluorescence for the A29V and I41T mutants.