Figure 3: Photophysical characterization of the oxazine fluorophore library.

(a) Tabulated photophysical properties of library, including wavelength of absorption and emission maximum, molar extinction coefficient, quantum yield and brightness, measured in phosphate buffered saline. (b) Normalized absorption spectra of 25–25, 25–27, and the 700 nm clinically relevant analog, methylene blue. (c) Normalized emission spectra of 25–25, 25–27, and the 700 nm clinically relevant analog, methylene blue.