Figure 4: In vivo white matter tract specificity and intensity screening of the BBB-permeable oxazine fluorophore library.

(a) Representative white light and fluorescence images of the whole brain and coronal after administration of 25–25, images are displayed with autocontrast adjustment for visualization. Labels present on coronal section images are for gray and white matter with 2.5 mm scale bars. (b) Average white matter tract in vivo intensity for all 18 fluorophores and a 4-hour timepoint vehicle-injected control following intravenous administration, n=4. (c) Average white-matter-to-gray-matter tissue intensity ratios (SBRs) for all 18 fluorophores and a 4-hour timepoint vehicle-injected control following intravenous administration, n=4. Average SBRs were compared to the vehicle-injected control with a one-way ANOVA using Geisser-Greenhouse correction followed by a Fisher’s LSD multiple comparison test, where 1, 2, 3 and 4 asterisks represent P values less than 0.05, 0.01, 0.001, and 0.0001 respectively. (d) Scatter plot of average white-matter-to-gray-matter tissue intensity ratio against the average normalized in vivo intensity for all 18 fluorophores and a vehicle injected control following intravenous administration.