Table 2. Estimated membrane (MB)-associated stoichiometry of RNAD components in rifampicin-treated cells.

As in Table 1, published protein abundance values from ribosome profiling (13) were converted to functional oligomeric forms and multiplied by the membrane-bound fraction (MB%) measured from rif-treated cells. MB% values are shown as the mean ± s.d. of the MCMC posterior distribution. MB-associated abundances were normalized to RNE, and errors represent uncertainty propagated from MB% estimation.

	MB%	Abundance from EZRDM		Abundance from MOPS glucose
		Multimer	MB-associated multimers, relative to RNE	Multimer	MB-associated multimers, relative to RNE
RNE4	98.0 ± 0.5	682	1	265	1
RhlB	99.6 ± 0.4	3,459	5.2 ± 0.0	1,055	4.0 ± 0.0
PNP3	91.7 ± 2.7	3,968	5.4 ± 0.1	1,181	4.2 ± 0.1
Eno2	0.7 ± 0.7	27,111	0.3 ± 0.3	10,617	0.3 ± 0.3