Table 1:
Cryo-EM data collection, refinement, and validation statistics.
| RNA polymerase II-DSIF-NELF-ELOF1 elongation complex (EMD-XXXX) (PDB: XXXX) |
|
|---|---|
| Data collection and processing | |
| Magnification | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 50.1 |
| Defocus range (μm) | 0.25-2.25 |
| Pixel size (Å) | 0.822 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 8,251,315 |
| Final particle images (no.) | 84,105 |
| Map resolution (Å) | 2.7 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.7-8 |
| Refinement | |
| Initial models used (PDB code) | 9BZ0, 8UHD, and 3H7H |
| Map sharpening B factor (Å2) | −15 |
| Model composition | |
| Non-hydrogen atoms | 94008 |
| Protein residues | 5756 |
| Nucleotides | 87 |
| Ligands | 11 |
| B factors (Å2) | |
| Protein | 111.63 |
| Nucleotide | 228.03 |
| Ligand | 183.23 |
| R.M.S. deviations | |
| Bond lengths (Å) | 0.003 |
| Bond angles (°) | 0.517 |
| Validation | |
| MolProbity score | 1.5 |
| Clashscore | 4.87 |
| Poor rotamers (%) | 0.71 |
| Ramachandran plot | |
| Favored (%) | 96.32 |
| Allowed (%) | 3.68 |
| Disallowed (%) | 0 |