Table 3.

Kinetic parameters of pairwise competiton experiments

Substrate	Km (μM)	Vmax (nMsec−1)
ssRNA vs PT-ssDNA (Ki = 0.12 ± 0.01μM) [Competitive]
0	0.09 ± 0.01	0.39 ± 0.01
0.375	0.33 ± 0.03	0.364 ± 0.009
0.75	0.55 ± 0.04	0.36 ± 0.01
1.5	1.5 ± 0.2	0.46 ± 0.02
3.0	2.7 ± 0.3	0.44 ± 0.02
ssDNA vs PT-ssRNA (Ki = 0.61 ± 0.09μM) [Competitive]
0.0	0.025 ± 0.007	0.185 ± 0.004
0.375	0.09 ± 0.01	0.199 ± 0.004
0.75	0.18 ± 0.02	0.209 ± 0.005
1.5	0.21 ± 0.02	0.196 ± 0.004
3.0	0.34 ± 0.02	0.214 ± 0.005
ssDNA vs PT-ssDNA(Ki = 0.09 ± 0.02μM)[Competitive]
0.0	0.025 ± 0.007	0.185 ± 0.004
0.75	0.24 ± 0.04	0.196 ± 0.008
1.5	0.46 ± 0.06	0.188 ± 0.007
3.0	0.71 ± 0.07	0.169 ± 0.006
dsDNA vs PT-ssDNA (Ki = 0.05 ± 0.01μM and Ki'= 0.7 ± 0.2μM) [Mixed]
0.0	0.042 ± 0.006	0.180 ± 0.005
0.09375	0.09 ± 0.02	0.17 ± 0.01
0.1875	0.20 ± 0.03	0.147 ± 0.007
0.75	0.27 ± 0.07	0.087 ± 0.009
dsDNA vs PT-ssRNA (Ki = 0.11 ± 0.03μM and Ki'= 0.9 ± 0.2μM) [Mixed]
0.0	0.042 ± 0.006	0.180 ± 0.005
0.375	0.11 ± 0.03	0.114 ± 0.007
0.75	0.17 ± 0.04	0.107 ± 0.009