|
Antibodies
|
| Anti-rabbit IgG (HRP) |
Santa Cruz Biotechnology |
Cat# sc-2357 |
| CD3 Pacific Blue |
BD Biosciences |
Cat# 558214 |
| CD4 APC |
BioLegend |
Cat# 100412 |
| CD4 Brilliant Violet 605 |
BioLegend |
Cat# 100451 |
| CD8 Alexa Fluor 700 |
ThermoFisher (Invitrogen) |
Cat# 56-0081-82 |
| CD11b Alexa Fluor 594 |
BioLegend |
Cat# 103254 |
| CD11b Alexa Fluor 647 |
BioLegend |
Cat# 101218 |
| CD11b Brilliant Violet 785 |
BioLegend |
Cat# 101243 |
| CD11c Brilliant Violet 650 |
BioLegend |
Cat# 117339 |
| CD19 Brilliant Ultra Violet 395 |
BD Biosciences |
Cat# 563557 |
| CD44 Brilliant Violet 785 |
BioLegend |
Cat# 103059 |
| CD47 FITC |
BioLegend |
Cat# 127503 |
| CD62L PE/Cy7 |
BioLegend |
Cat# 104418 |
| CD64 Brilliant Violet 605 |
BioLegend |
Cat# 139323 |
| CD71 APC/Fire750 |
BioLegend |
Cat# 113828 |
| CD80 Brilliant Violet 421 |
BioLegend |
Cat# 104726 |
| CD86 Brilliant Violet 421 |
BioLegend |
Cat# 105123 |
| CXCL9 Alexa Fluor 647 |
BioLegend |
Cat# 515606 |
| GAPDH |
Santa Cruz Biotechnology |
Cat# sc-32233 |
| IFNγ APC |
BioLegend |
Cat# 505810 |
| IL-4Ra PE/Cy7 |
BioLegend |
Cat# 144806 |
| IL-6 APC |
BioLegend |
Cat# 504508 |
| IL-10 APC/Cy7 |
Biolegend |
Cat# 505035 |
| IL-17a Brilliant Violet 650 |
BioLegend |
Cat# 506930 |
| IL-18Rα PE |
BioLegend |
Cat# 157904 |
| iNOS Brilliant Ultra Violet 737 |
Thermo Fisher (Invitrogen) |
Cat# 367-5920-82 |
| Ly6C PerCP/Cy5.5 |
Thermo Fisher (Invitrogen) |
Cat# 45-5932-82 |
| Ly6G Brilliant Ultra Violet 395 |
BD Biosciences |
Cat# 563978 |
| MerTK APC/eFluor780 |
Thermo Fisher (Invitrogen) |
Cat# 47-5751-82 |
| MHC-II Alexa Fluor 700 |
BioLegend |
Cat# 107622 |
| PD-L1 Biotin |
BioLegend |
Cat# 124306 |
| Salmonella (CSA-1) FITC |
Seracare |
Cat# 5310-0322 |
| SIRP-α APC/Cy7 |
BioLegend |
Cat# 144017 |
| SPIC |
Thermo Fisher (Invitrogen) |
Cat# PA5-106516 |
| Ter119 PE |
Biolegend |
Cat# 116208 |
| TGF-β Brilliant Violet 421 |
BioLegend |
Cat# 141408 |
| TNF-α Brilliant Violet 510 |
BioLegend |
Cat# 506339 |
| VCAM1 Alexa Fluor 488 |
BioLegend |
Cat# 105710 |
| VCAM1 Biotin |
BioLegend |
Cat# 105704 |
| VCAM1 PE/Cy7 |
BioLegend |
Cat# 105720 |
|
Chemicals, Peptides, and Recombinant Proteins
|
| ACK lysis buffer |
Thermofisher (Gibco) |
Cat# A10492-01 |
| BD cytofix/cytoperm solution kit |
BD Biosciences |
Cat# 554714 |
| DAPI |
ThermoFisher (Invitrogen) |
Cat# D1306 |
| DNase I |
Sigma |
Cat# 10104159001 |
| GoTaq PCR Master Mix |
Promega |
Cat# M7123 |
| Liberase TL |
Sigma |
Cat# 05401020001 |
| Live/Dead Fixable Blue |
ThermoFisher (Invitrogen) |
Cat# L23105
|
| Luna Universal Probe qPCR Master Mix |
New England Biolabs |
Cat# M3004 |
| Mouse IgG1-binding protein (HRP) |
Santa Cruz Biotechnology |
Cat# sc-525408 |
| OCT |
Fisher Healthcare |
Cat# 23-730-571 |
| ProLong Diamond Antifade Mountant |
ThermoFisher (Invitrogen) |
P36961 |
| Streptavidin Brilliant Violet 510 |
BioLegend |
Cat# 405234 |
| TruStain fcX |
BioLegend |
Cat# 101320 |
|
Experimental Models: Organisms/Strains
|
| 129X1/SvJ mice |
Jackson Laboratory and inhouse colony |
Stock #: 000691 |
| 129X1/SvJ mice, SPIC KO |
This study |
|
|
Bacteria Strains
|
| Salmonella enterica serovar Typhimurium, strain SL1344, StrepR |
Gift from Dr. Denise Monack, Stanford University |
N/A |
| Salmonella enterica serovar Typhimurium SL1344 Tomato, StrepR |
Gift from Dr. Marc Jenkins, University of Minnesota |
N/A |
|
Oligonucleotides and Probes
|
| SPIC Outer Forward Primer (Crude amplification) |
IDT (Custom-made) |
5’-CCGTAGAGGGAATGGGTTATG-3’ |
| SPIC Outer Reverse Primer (Crude amplification) |
IDT (Custom-made) |
5’-CTTCCTCTCTCTGCTGCTATTT-3’ |
| SPIC Inner Forward Primer (qPCR assay) |
IDT (Custom-made) |
5’-GGTTGCTTACAGATGTGTTCATA- |
| SPIC Inner Reverse Primer (qPCR assay) |
IDT (Custom-made) |
5’-TGACATTCCATAGTAGTTAGCG-3’. |
| SPIC WT Hydrolysis Probe (qPCR assay) |
IDT (PrimeTime, Custom-made,) |
5’-TATCCTCACGTCAGAGGCAA-3’, FAM/Zen/Iowa Black FQ modified |
| SPIC KO Hydrolysis Probe (qPCR assay) |
IDT (PrimeTime, Custom-made,) |
5’-CAATCCGTACAGAGGCAACG-3’ SUN/ZEN/Iowa Black FQ modified |
|
Software
|
| FlowJo |
BD Biosciences |
https://www.flowjo.com
|
| FIJI |
Schindelin et al., 2012 |
https://imagej.nih.gov/ij/
|
| Prism |
GraphPad Software Inc. |
https://www.graphpad.com/scientific-software/prism/
|
