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. 2006 Jan;18(1):15–28. doi: 10.1105/tpc.105.037200

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Copyright © 2006, American Society of Plant Biologists

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Figure 5. — Phenotypes of osmads58RNAi Flowers.

(A) osmads58-s1 flower. Arrow indicates a whorl 3 stamen that has been partially transformed into a lodicule.

(B) osmads58-s1 flower. Arrow indicates an ectopic cell mass emerging from a carpel-like organ.

(C) osmads58-w1 flower. Arrow indicates an ectopic stamen produced interior to the multiple carpels in whorl 4.

(D) osmads58-w1 flower. Arrow indicates a carpel-like organ, similar to that formed in osmads58-s1. Arrowheads in (A) to (D) indicate ectopic lodicules in whorl 2.

(E) and (F) Scanning electron micrographs of the osmads58-s1 flower showing the reiterative development of lodicules, stamens, and carpel-like organs. Some organs in the outer whorls have been removed to show indeterminate organ development. Arrow in (E) indicates a partially transformed stamen into the lodicule. In (F), each floral organ is indicated by a different color: purple, lodicule; yellow, stamen; green, carpel-like organ.

(G) Scanning electron micrograph of the osmads58-s1 flower at anthesis. Some organs in the outer whorls have been removed to show indeterminate organ development.

(H) Close-up of (G). The floral meristem (fm), which still produces organs, can be observed.

(I) Scanning electron micrograph of a carpel-like organ in osmads58-s1.

(J) and (K) Scanning electron micrographs showing the epidermal morphology of a wild-type carpel (J) and the carpel-like organ of the osmads58-s1 flower (K).

(L) Scanning electron micrograph of trichomes in the carpel-like organs of the osmads58-s1 flower.

(M) Scanning electron micrograph of bristles in wild-type lemma.

(N) Scanning electron micrograph of an early stage of the osmads58-s1 flower.

(O) Longitudinal section of the carpel-like organ showing an ovule-like structure (ovl) in the osmads58-s1 flower.

(P) and (Q) In situ localization of OSH1 transcripts in late stage of the wild-type (P) and osmads58-s1 (Q) flowers. Asterisks in (Q) indicate floral organ primordia.

ca, carpel; cl, carpel-like organ; cm, cell mass; elo, ectopic lodicule; est, ectopic stamen developing reiteratively; lo, original lodicule; ov, ovule. Bars = 500 μm in (E) to (I) and 20 μm in (J) to (Q).