Figure 1.

ror1 Mutations Release the TGS of the Pro_35S:NPTII Transgene in the ros1 Mutant.

(A) ror1-1 ros1 and ror1-2 ros1 mutants containing the Pro_35S:NPTII reporter transgene exhibit similar kanamycin-resistant phenotypes as C24 wild-type plants, whereas the ros1 mutant is sensitive to kanamycin.

(B) and (C) Comparison of luminescence after ABA treatment for 5 h in ror1-1 ros1, ror1-2 ros1, ros1, and C24 seedlings.

(B) C24 wild type (top left), ros1 (top right), ror1-1 ros1 (bottom left), and ror1-2 ros1 (bottom right) seedlings grown on MS medium.

(C) Luminescence comparison of different plants in (B).

(D) F1 seedlings of ror1 ros1 crossed with ros1 are sensitive to kanamycin. C24 wild type (top left), ros1 (top right), ror1-1 ros1 (bottom left), and F1 (crossed between ror1-1 ros1 and ros1, bottom right) seedlings grown on MS medium supplemented with 50 mg/L kanamycin.

(E) Seedlings of selfed F1 progeny segregate at a ratio of 3:1 kanamycin-sensitive:kanamycin-resistant seedlings. Plants were grown on MS medium containing 50 mg/L kanamycin.