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. 2006 Jan;18(1):85–103. doi: 10.1105/tpc.105.037507

Figure 2.

Figure 2.

ror1 Mutations Have No Effect on Expression of Either RD29A or LUC but Increase the Expression of NPTII and TSI Transcripts.

(A) RNA gel blot analysis with RD29A and LUC as probes reveals that RD29A and LUC remain silenced in ror1-1 ros1 and ros1 mutants after ABA treatment. By contrast, RD29A and LUC expression was induced to a high level in C24 wild-type plants. COR47 was used as a positive control for ABA treatment. Tubulin was used as a loading control.

(B) RT-PCR analysis of NTPII expression. NPTII transcripts were detected in both ror1 ros1 and the wild type but not in ros1.

(C) RNA gel blot analysis with the TSI fragment as a probe shows that ror1 mutations increase the level of TSI transcripts. The expression of TSI in ror1-3, a T-DNA insertion mutant, was compared with that in the wild type. ddm1 and ddm1 ros1 mutants were used as positive controls for TSI expression. Tubulin was used as a loading control. Col, Columbia accession.