Figure 9.

Interaction of ROR1/RPA2A and ROS1 in a Yeast Two-Hybrid System and ROR1/RPA2A-GFP Cellular Localization.

(A) ROR1/RPA2A-ROS1 interaction in a yeast two-hybrid assay. Full-length ROR1/RPA2A cDNA (840 bp) was fused to the GAL4 activation domain in the pACT2 vector. Different parts of ROS1 cDNA were fused with the GAL4 DNA binding domain in pGBKT7 vector. Interaction was observed when ROR1/RPA2A was cotransformed with full-length ROS1 or the C terminus (506 to 1391) of ROS1. ROR1/RPA2A or ROS1 did not show any activity when cotransformed with empty vector.

(B) T-DNA structure used for transient ROR1/RPA2A-GFP expression. Hyg, hygromycin-resistant gene.

(C) Transient expression of the ROR1/RPA2A-GFP fusion protein in onion epidermal cells under confocal microscopy. ROR1/RPA2A-GFP was localized in the nucleus.

(D) Bright-field image of (C).

(E) Transient expression of the GFP protein in onion epidermal cells. Compared with localization of ROR1/RPA2-GFPA fused protein, GFP is more distributed in the cytoplasm.

(F) Bright-field image of (E). Bars = 100 μm.