Skip to main content
. 2006 Jan;18(1):176–197. doi: 10.1105/tpc.105.036392

Table 1.

Identified Proteins from Arabidopsis and Mustard in TAC Preparations

ΔMd
xcorre
NCBI Acc. No./AGI Acc. No.a Protein Positionb Zc Ara Sin Ara Sin
Transcription
gi|7525065 RNA polymerase α-chain (RpoA) 40–50 2 0.7 2.60
ArthCp055 292–310 2 0.6 1.2 6.62 3.87
322–328 2 −0.1 2.60
gi|7525025 RNA polymerase β-chain (RpoB) 13–31 2 −0.2 4.30
ArthCp014 32–43 2 0.0 3.95
276–290 2 0.4 0.0 4.36 3.44
322–337 2 0.1 0.5 4.98 4.08
354–386 3 0.7 3.60
387–397 2 0.7 3.47
450–466 2 −0.6 4.11
603–621 2 −0.4 3.16
643–661 2 −0.2 3.44
769–779 2 −0.2 3.06
907–917 2 0.1 3.79
918–932 2 −1.1 4.64
940–957 2 0.3 0.1 3.57 3.87
993–1017 3 0.0 0.1 4.73 4.04
1025–1048 2 1.4 2.72
1056–1069 2 0.3 0.0 5.12 4.30
gi|7525024 RNA polymerase β′-chain (RpoC1) 12–26 2 0.2 3.26
ArthCp013 173–188 2 1.0 3.34
287–297 2 0.4 3.60
303–314 2 0.6 3.54
327–341 2 0.3 2.69
382–394 2 −0.6 3.30
434–454 3 0.2 5.21
461–474 2 0.3 0.9 3.97 3.27
485–510 3 1.0 3.78
596–605 2 1.3 2.72
gi|7525023 RNA polymerase β″-chain (RpoC2) 44–68 2 0.5 0.8 3.30 3.62
ArthCp012 69–84 2 0.3 6.04
241–249 2 −0.8 0.1 2.98 2.57
250–260 2 0.3 −1.2 3.72 2.51
266–276 2 0.1 4.25
277–288 2 0.3 2.91
307–338 3 −1.0 3.02
434–454 3 0.2 5.12
485–510 3 1.0 3.78
526–535 2 0.4 2.07
590–608 2 0.7 2.78
610–620 2 1.0 2.54
720–730 2 1.3 0.1 2.99 2.59
779–801 2 1.1 3.12
810–823 2 −0.6 2.96
824–834 2 0.7 −0.3 2.98 3.98
1152–1162 2 0.0 −1.2 2.61 3.55
1227–1248 3 0.1 5.12
1309–1325 2 0.3 3.92
1345–1354 2 0.3 2.92
DNA replication, DNA topology, DNA binding
gi|11994141 DNA polymerase A, putative (PolA) 287–302 2 0.3 3.92
At3g20540 322–337 2 0.3 3.24
342–356 2 0.6 4.12
377–391 2 0.5 4.11
448–466 2 −1.1 2.72
gi|15228353 DNA gyrase subunit A, putative (GyrA) 109–125 2 −0.3 −1.6 2.89 2.90
At3g10690 194–201 1 0.7 2.19
220–248 3 0.0 4.52
352–365 2 −0.9 3.91
405–417 2 0.6 3.79
444–453 2 −0.8 2.85
535–548 2 0.6 0.7 4.22 3.97
562–571 2 −1.6 −1.1 2.90 3.21
675–692 2 −1.7 3.91
gi|15228245 DNA gyrase subunit B, putative (GyrB) 368–394 3 −0.4 3.61
At3g10270 484–499 2 −1.6 −0.8 5.81 4.75
Translation
gi|15237059 Elongation factor Tu (EF-Tu) 127–142 2 0.5 4.22
At4g20360 205–222 2 0.5 2.97
283–301 2 −0.1 −0.1 4.42 3.19
316–326 2 0.4 0.1 3.44 3.07
342–357 2 −0.2 3.53
gi|15232274 Ribosomal protein L12-1 69–82 2 0.3 4.57
At3g27830 83–91 2 0.6 2.87
gi|5881732 Ribosomal protein S3 68–82 2 0.0 4.89
ArthCp061 92–101 2 0.5 3.61
148–158 2 −0.2 4.19
159–167 2 0.6 3.26
184–192 1 0.4 1.71
gi|15238369 Ribosomal protein L29 74–86 2 −0.1 2.82
At5g65220 97–103 1 1.2 1.68
Detoxification, protein modification
gi|15241373 Iron superoxide dismutase (FE-SOD1) 92–113 2 0.6 5.80
At5g51100 155–165 2 0.0 3.77
168–184 2 1.1 4.03
188–199 2 −0.8 0.2 4.28 3.67
210–241 3 −0.4 0.9 4.72 4.2
243–252 2 0.0 3.15
253–262 2 0.3 0.0 2.97 3.17
gi|11358867 Iron superoxide dismutase (FE-SOD3) 96–106 2 0.7 3.33
At5g23310 145–161 3 −1.5 3.83
228–245 3 −1.5 3.68
gi|15230779 Thioredoxin, putative 80–90 2 0.5 −1.0 3.58 3.41
At3g06730 132–142 2 0.3 0.4 3.14 3.40
166–183 2 0.8 2.91
188–199 2 0.1 3.65
Metabolism
gi|15222232 pfkB-type carbohydrate kinase family protein (PFKB1) 89–119 3 −1.5 4.75
At1g69200 227–235 2 0.7 2.66
334–342 2 0.1 2.87
396–412 2 0.6 4.20
437–454 2 0.8 3.96
490–504 2 1.1 0.4 2.80 4.23
505–516 2 0.6 −0.6 3.22 2.52
601–614 2 0.5 3.83
gi|15232415 pfkB-type carbohydrate kinase family protein (PFKB2) 146–160 3 −0.5 3.05
At3g54090 223–240 2 −0.8 3.46
327–341 2 0.6 4.32
364–373 2 0.0 3.04
401–413 2 −0.5 3.93
gi|15222509 Mur ligase family protein 213–231 2 0.2 3.34
At1g63680 252–273 2 −1.5 2.86
318–338 2 0.3 6.31
389–409 2 1.0 3.51
746–767 2 −0.1 3.43
DNA/RNA binding, unknown function
gi|15223748 PTAC1 (DNA binding protein p24-related) 64–80 2 0.5 3.20
At1g14410 81–89 2 0.7 2.91
100–112 2 0.8 2.60
138–156 2 0.8 3.36
181–199 2 0.2 0.7 3.10 3.99
200–213 2 0.6 3.64
214–247 3 0.1 3.73
248–263 2 0.0 −1.4 3.56 2.68
gi|15221411 At1g74850 PTAC2 (pentatricopeptide repeat-containing protein) 57–69 2 0.4 3.15
103–113 2 −0.2 0.8 3.77 3.76
192–201 2 0.3 −0.5 3.37 3.18
224–239 2 0.6 4.20
718–740 2 0.4 2.98
741–753 2 0.4 3.48
gi|15229259 PTAC3 (SAP domain-containing protein) 74–83 2 0.0 3.30
At3g04260 88–109 2 −0.3 4.90
178–189 2 0.1 4.59
162–171 2 0.9 2.92
304–319 2 −0.3 3.86
353–376 2 1.4 4.77
384–395 2 0.4 0.0 4.25 3.01
414–438 2 0.6 5.10
459–479 2 0.8 5.30
496–515 2 1.1 3.55
547–560 2 0.6 3.43
618–631 2 1.0 0.7 4.27 3.82
703–713 2 1.0 4.30
gi|18408237 PTAC4 (PspA/IM30 family protein) 84–98 2 0.7 3.10
At1g65260 99–113 2 −0.4 5.19
116–126 2 0.2 3.54
146–152 1 0.2 1.71
166–176 2 0.1 3.47
167–176 2 −0.5 3.43
184–194 2 −0.1 3.13
223–241 2 0.5 5.91
246–267 2 1.1 5.08
268–284 3 −0.5 4.35
313–324 2 0.1 4.58
gi|15236331 At4g13670 PTAC5 (peptidoglycan binding domain-containing protein) 80–92 2 −0.1 −0.6 4.78 4.40
221–229 2 −0.1 2.96
gi|18395008 PTAC6 (expressed protein) 83–112 2 1.1 6.16
At1g21600 113–129 2 −0.3 3.21
149–161 2 1.0 3.06
162–177 2 1.1 2.84
232–250 2 0.8 5.19
gi|22327025 PTAC7 (expressed protein) 64–72 2 0.6 2.73
At5g24314 80–91 2 −0.8 4.50
96–112 2 0.9 2.80
123–131 2 −0.9 2.80
132–161 2 0.7 4.79
gi|18407178 PTAC8 (expressed protein) 64–88 2 1.0 4.80
At2g46820 127–148 2 −0.2 3.96
gi|18415421 PTAC9 (expressed protein) 121–130 2 1.5 0.0 2.63 3.59
At4g20010 288–299 2 0.7 3.47
gi|15228384 PTAC10 (S1 domain-containing protein) 41–56 2 0.7 −0.4 3.36 4.10
At3g48500 77–93 2 −1.1 0.0 3.87 4.15
95–114 2 0.2 4.61
127–138 2 0.9 3.04
194–201 1 0.7 2.19
244–252 2 0.2 2.67
354–362 2 −0.3 2.69
404–424 2 0.6 5.64
444–453 2 0.4 3.35
468–478 2 0.1 3.86
503–515 2 −0.3 4.35
532–543 2 0.3 0.4 3.22 3.21
553–571 2 −0.3 5.68
gi|15227028 PTAC11 (DNA binding protein p24-related) 69–84 2 0.7 3.02
At2g02740 81–89 2 0.7 2.91
96–103 2 0.0 2.58
104–116 2 0.4 0.4 3.83 3.78
120–134 2 0.1 −0.6 3.73 4.33
133–146 2 0.8 3.99
196–204 2 0.1 0.1 3.34 3.26
205–218 2 0.3 −0.6 3.23 5.77
219–252 3 0.6 3.76
gi|15226791 PTAC12 (unknown protein) 106–121 2 0.6 2.66
At2g34640 127–142 2 0.6 4.41
190–206 2 0.1 3.03
225–236 2 0.4 3.28
250–259 2 0.4 3.28
269–283 2 −0.1 4.55
280–294 2 0.3 4.79
304–315 2 −0.4 2.70
329–351 3 0.2 4.88
340–362 3 0.2 4.65
gi|18398425At3g09210 PTAC13 (KOW domain-containing transcription factor family protein) 65–70 1 −0.9 1.69
122–138 2 −0.5 3.05
174–185 2 0.6 2.51
198–209 2 −0.7 3.97
285–298 2 0.1 4.11
gi|42566980 PTAC14 (SET domain-containing protein) 118–131 2 0.8 3.29
At4g20130 224–230 1 0.6 1.70
250–260 2 0.1 2.92
337–350 2 −0.3 3.61
357–383 3 0.5 5.15
384–397 2 0.2 4.61
398–415 2 1.0 2.72
gi|15239573At5g54180 PTAC15 (mitochondrial transcription termination factor-related) 222–237 2 −0.2 4.15
259–273 2 1.3 3.57
347–361 2 0.7 3.70
gi|42565672 PTAC16 (expressed protein) 120–130 2 0.2 3.10
At3g46780 149–164 2 0.3 3.13
237–248 2 0.8 2.87
249–260 2 −1.9 −0.1 4.42 2.68
gi|15220146At1g80480 PTAC17 (cobW domain-containing protein) 89–102 2 0.4 2.66
208–218 2 −1.7 3.10
gi|15225202 PTAC18 (expressed protein) 75–93 2 0.7 −1.1 4.00 4.64
At2g32180 116–130 2 0.5 5.68

Analysis of tandem MS (MS/MS) spectra is based on the Arabidopsis database (ftp://ftp.arabidopsis.org/home/tair/sequences/blast_datasets/).

a

National Center for Biotechnology Information/Arabidopsis Genome Initiative accession numbers.

b

Position of identified peptides within the database protein sequence.

c

The charge state of the measured ion (z).

d

The calculated deviation of the experimentally determined mass from the theoretical average mass of the peptide. Ara, Arabidopsis; Sin, S. alba.

e

xcorr value calculated using the sequest algorithm.