Table 1.
ΔMd
|
xcorre
|
||||||
---|---|---|---|---|---|---|---|
NCBI Acc. No./AGI Acc. No.a | Protein | Positionb | Zc | Ara | Sin | Ara | Sin |
Transcription | |||||||
gi|7525065 | RNA polymerase α-chain (RpoA) | 40–50 | 2 | 0.7 | 2.60 | ||
ArthCp055 | 292–310 | 2 | 0.6 | 1.2 | 6.62 | 3.87 | |
322–328 | 2 | −0.1 | 2.60 | ||||
gi|7525025 | RNA polymerase β-chain (RpoB) | 13–31 | 2 | −0.2 | 4.30 | ||
ArthCp014 | 32–43 | 2 | 0.0 | 3.95 | |||
276–290 | 2 | 0.4 | 0.0 | 4.36 | 3.44 | ||
322–337 | 2 | 0.1 | 0.5 | 4.98 | 4.08 | ||
354–386 | 3 | 0.7 | 3.60 | ||||
387–397 | 2 | 0.7 | 3.47 | ||||
450–466 | 2 | −0.6 | 4.11 | ||||
603–621 | 2 | −0.4 | 3.16 | ||||
643–661 | 2 | −0.2 | 3.44 | ||||
769–779 | 2 | −0.2 | 3.06 | ||||
907–917 | 2 | 0.1 | 3.79 | ||||
918–932 | 2 | −1.1 | 4.64 | ||||
940–957 | 2 | 0.3 | 0.1 | 3.57 | 3.87 | ||
993–1017 | 3 | 0.0 | 0.1 | 4.73 | 4.04 | ||
1025–1048 | 2 | 1.4 | 2.72 | ||||
1056–1069 | 2 | 0.3 | 0.0 | 5.12 | 4.30 | ||
gi|7525024 | RNA polymerase β′-chain (RpoC1) | 12–26 | 2 | 0.2 | 3.26 | ||
ArthCp013 | 173–188 | 2 | 1.0 | 3.34 | |||
287–297 | 2 | 0.4 | 3.60 | ||||
303–314 | 2 | 0.6 | 3.54 | ||||
327–341 | 2 | 0.3 | 2.69 | ||||
382–394 | 2 | −0.6 | 3.30 | ||||
434–454 | 3 | 0.2 | 5.21 | ||||
461–474 | 2 | 0.3 | 0.9 | 3.97 | 3.27 | ||
485–510 | 3 | 1.0 | 3.78 | ||||
596–605 | 2 | 1.3 | 2.72 | ||||
gi|7525023 | RNA polymerase β″-chain (RpoC2) | 44–68 | 2 | 0.5 | 0.8 | 3.30 | 3.62 |
ArthCp012 | 69–84 | 2 | 0.3 | 6.04 | |||
241–249 | 2 | −0.8 | 0.1 | 2.98 | 2.57 | ||
250–260 | 2 | 0.3 | −1.2 | 3.72 | 2.51 | ||
266–276 | 2 | 0.1 | 4.25 | ||||
277–288 | 2 | 0.3 | 2.91 | ||||
307–338 | 3 | −1.0 | 3.02 | ||||
434–454 | 3 | 0.2 | 5.12 | ||||
485–510 | 3 | 1.0 | 3.78 | ||||
526–535 | 2 | 0.4 | 2.07 | ||||
590–608 | 2 | 0.7 | 2.78 | ||||
610–620 | 2 | 1.0 | 2.54 | ||||
720–730 | 2 | 1.3 | 0.1 | 2.99 | 2.59 | ||
779–801 | 2 | 1.1 | 3.12 | ||||
810–823 | 2 | −0.6 | 2.96 | ||||
824–834 | 2 | 0.7 | −0.3 | 2.98 | 3.98 | ||
1152–1162 | 2 | 0.0 | −1.2 | 2.61 | 3.55 | ||
1227–1248 | 3 | 0.1 | 5.12 | ||||
1309–1325 | 2 | 0.3 | 3.92 | ||||
1345–1354 | 2 | 0.3 | 2.92 | ||||
DNA replication, DNA topology, DNA binding | |||||||
gi|11994141 | DNA polymerase A, putative (PolA) | 287–302 | 2 | 0.3 | 3.92 | ||
At3g20540 | 322–337 | 2 | 0.3 | 3.24 | |||
342–356 | 2 | 0.6 | 4.12 | ||||
377–391 | 2 | 0.5 | 4.11 | ||||
448–466 | 2 | −1.1 | 2.72 | ||||
gi|15228353 | DNA gyrase subunit A, putative (GyrA) | 109–125 | 2 | −0.3 | −1.6 | 2.89 | 2.90 |
At3g10690 | 194–201 | 1 | 0.7 | 2.19 | |||
220–248 | 3 | 0.0 | 4.52 | ||||
352–365 | 2 | −0.9 | 3.91 | ||||
405–417 | 2 | 0.6 | 3.79 | ||||
444–453 | 2 | −0.8 | 2.85 | ||||
535–548 | 2 | 0.6 | 0.7 | 4.22 | 3.97 | ||
562–571 | 2 | −1.6 | −1.1 | 2.90 | 3.21 | ||
675–692 | 2 | −1.7 | 3.91 | ||||
gi|15228245 | DNA gyrase subunit B, putative (GyrB) | 368–394 | 3 | −0.4 | 3.61 | ||
At3g10270 | 484–499 | 2 | −1.6 | −0.8 | 5.81 | 4.75 | |
Translation | |||||||
gi|15237059 | Elongation factor Tu (EF-Tu) | 127–142 | 2 | 0.5 | 4.22 | ||
At4g20360 | 205–222 | 2 | 0.5 | 2.97 | |||
283–301 | 2 | −0.1 | −0.1 | 4.42 | 3.19 | ||
316–326 | 2 | 0.4 | 0.1 | 3.44 | 3.07 | ||
342–357 | 2 | −0.2 | 3.53 | ||||
gi|15232274 | Ribosomal protein L12-1 | 69–82 | 2 | 0.3 | 4.57 | ||
At3g27830 | 83–91 | 2 | 0.6 | 2.87 | |||
gi|5881732 | Ribosomal protein S3 | 68–82 | 2 | 0.0 | 4.89 | ||
ArthCp061 | 92–101 | 2 | 0.5 | 3.61 | |||
148–158 | 2 | −0.2 | 4.19 | ||||
159–167 | 2 | 0.6 | 3.26 | ||||
184–192 | 1 | 0.4 | 1.71 | ||||
gi|15238369 | Ribosomal protein L29 | 74–86 | 2 | −0.1 | 2.82 | ||
At5g65220 | 97–103 | 1 | 1.2 | 1.68 | |||
Detoxification, protein modification | |||||||
gi|15241373 | Iron superoxide dismutase (FE-SOD1) | 92–113 | 2 | 0.6 | 5.80 | ||
At5g51100 | 155–165 | 2 | 0.0 | 3.77 | |||
168–184 | 2 | 1.1 | 4.03 | ||||
188–199 | 2 | −0.8 | 0.2 | 4.28 | 3.67 | ||
210–241 | 3 | −0.4 | 0.9 | 4.72 | 4.2 | ||
243–252 | 2 | 0.0 | 3.15 | ||||
253–262 | 2 | 0.3 | 0.0 | 2.97 | 3.17 | ||
gi|11358867 | Iron superoxide dismutase (FE-SOD3) | 96–106 | 2 | 0.7 | 3.33 | ||
At5g23310 | 145–161 | 3 | −1.5 | 3.83 | |||
228–245 | 3 | −1.5 | 3.68 | ||||
gi|15230779 | Thioredoxin, putative | 80–90 | 2 | 0.5 | −1.0 | 3.58 | 3.41 |
At3g06730 | 132–142 | 2 | 0.3 | 0.4 | 3.14 | 3.40 | |
166–183 | 2 | 0.8 | 2.91 | ||||
188–199 | 2 | 0.1 | 3.65 | ||||
Metabolism | |||||||
gi|15222232 | pfkB-type carbohydrate kinase family protein (PFKB1) | 89–119 | 3 | −1.5 | 4.75 | ||
At1g69200 | 227–235 | 2 | 0.7 | 2.66 | |||
334–342 | 2 | 0.1 | 2.87 | ||||
396–412 | 2 | 0.6 | 4.20 | ||||
437–454 | 2 | 0.8 | 3.96 | ||||
490–504 | 2 | 1.1 | 0.4 | 2.80 | 4.23 | ||
505–516 | 2 | 0.6 | −0.6 | 3.22 | 2.52 | ||
601–614 | 2 | 0.5 | 3.83 | ||||
gi|15232415 | pfkB-type carbohydrate kinase family protein (PFKB2) | 146–160 | 3 | −0.5 | 3.05 | ||
At3g54090 | 223–240 | 2 | −0.8 | 3.46 | |||
327–341 | 2 | 0.6 | 4.32 | ||||
364–373 | 2 | 0.0 | 3.04 | ||||
401–413 | 2 | −0.5 | 3.93 | ||||
gi|15222509 | Mur ligase family protein | 213–231 | 2 | 0.2 | 3.34 | ||
At1g63680 | 252–273 | 2 | −1.5 | 2.86 | |||
318–338 | 2 | 0.3 | 6.31 | ||||
389–409 | 2 | 1.0 | 3.51 | ||||
746–767 | 2 | −0.1 | 3.43 | ||||
DNA/RNA binding, unknown function | |||||||
gi|15223748 | PTAC1 (DNA binding protein p24-related) | 64–80 | 2 | 0.5 | 3.20 | ||
At1g14410 | 81–89 | 2 | 0.7 | 2.91 | |||
100–112 | 2 | 0.8 | 2.60 | ||||
138–156 | 2 | 0.8 | 3.36 | ||||
181–199 | 2 | 0.2 | 0.7 | 3.10 | 3.99 | ||
200–213 | 2 | 0.6 | 3.64 | ||||
214–247 | 3 | 0.1 | 3.73 | ||||
248–263 | 2 | 0.0 | −1.4 | 3.56 | 2.68 | ||
gi|15221411 At1g74850 | PTAC2 (pentatricopeptide repeat-containing protein) | 57–69 | 2 | 0.4 | 3.15 | ||
103–113 | 2 | −0.2 | 0.8 | 3.77 | 3.76 | ||
192–201 | 2 | 0.3 | −0.5 | 3.37 | 3.18 | ||
224–239 | 2 | 0.6 | 4.20 | ||||
718–740 | 2 | 0.4 | 2.98 | ||||
741–753 | 2 | 0.4 | 3.48 | ||||
gi|15229259 | PTAC3 (SAP domain-containing protein) | 74–83 | 2 | 0.0 | 3.30 | ||
At3g04260 | 88–109 | 2 | −0.3 | 4.90 | |||
178–189 | 2 | 0.1 | 4.59 | ||||
162–171 | 2 | 0.9 | 2.92 | ||||
304–319 | 2 | −0.3 | 3.86 | ||||
353–376 | 2 | 1.4 | 4.77 | ||||
384–395 | 2 | 0.4 | 0.0 | 4.25 | 3.01 | ||
414–438 | 2 | 0.6 | 5.10 | ||||
459–479 | 2 | 0.8 | 5.30 | ||||
496–515 | 2 | 1.1 | 3.55 | ||||
547–560 | 2 | 0.6 | 3.43 | ||||
618–631 | 2 | 1.0 | 0.7 | 4.27 | 3.82 | ||
703–713 | 2 | 1.0 | 4.30 | ||||
gi|18408237 | PTAC4 (PspA/IM30 family protein) | 84–98 | 2 | 0.7 | 3.10 | ||
At1g65260 | 99–113 | 2 | −0.4 | 5.19 | |||
116–126 | 2 | 0.2 | 3.54 | ||||
146–152 | 1 | 0.2 | 1.71 | ||||
166–176 | 2 | 0.1 | 3.47 | ||||
167–176 | 2 | −0.5 | 3.43 | ||||
184–194 | 2 | −0.1 | 3.13 | ||||
223–241 | 2 | 0.5 | 5.91 | ||||
246–267 | 2 | 1.1 | 5.08 | ||||
268–284 | 3 | −0.5 | 4.35 | ||||
313–324 | 2 | 0.1 | 4.58 | ||||
gi|15236331 At4g13670 | PTAC5 (peptidoglycan binding domain-containing protein) | 80–92 | 2 | −0.1 | −0.6 | 4.78 | 4.40 |
221–229 | 2 | −0.1 | 2.96 | ||||
gi|18395008 | PTAC6 (expressed protein) | 83–112 | 2 | 1.1 | 6.16 | ||
At1g21600 | 113–129 | 2 | −0.3 | 3.21 | |||
149–161 | 2 | 1.0 | 3.06 | ||||
162–177 | 2 | 1.1 | 2.84 | ||||
232–250 | 2 | 0.8 | 5.19 | ||||
gi|22327025 | PTAC7 (expressed protein) | 64–72 | 2 | 0.6 | 2.73 | ||
At5g24314 | 80–91 | 2 | −0.8 | 4.50 | |||
96–112 | 2 | 0.9 | 2.80 | ||||
123–131 | 2 | −0.9 | 2.80 | ||||
132–161 | 2 | 0.7 | 4.79 | ||||
gi|18407178 | PTAC8 (expressed protein) | 64–88 | 2 | 1.0 | 4.80 | ||
At2g46820 | 127–148 | 2 | −0.2 | 3.96 | |||
gi|18415421 | PTAC9 (expressed protein) | 121–130 | 2 | 1.5 | 0.0 | 2.63 | 3.59 |
At4g20010 | 288–299 | 2 | 0.7 | 3.47 | |||
gi|15228384 | PTAC10 (S1 domain-containing protein) | 41–56 | 2 | 0.7 | −0.4 | 3.36 | 4.10 |
At3g48500 | 77–93 | 2 | −1.1 | 0.0 | 3.87 | 4.15 | |
95–114 | 2 | 0.2 | 4.61 | ||||
127–138 | 2 | 0.9 | 3.04 | ||||
194–201 | 1 | 0.7 | 2.19 | ||||
244–252 | 2 | 0.2 | 2.67 | ||||
354–362 | 2 | −0.3 | 2.69 | ||||
404–424 | 2 | 0.6 | 5.64 | ||||
444–453 | 2 | 0.4 | 3.35 | ||||
468–478 | 2 | 0.1 | 3.86 | ||||
503–515 | 2 | −0.3 | 4.35 | ||||
532–543 | 2 | 0.3 | 0.4 | 3.22 | 3.21 | ||
553–571 | 2 | −0.3 | 5.68 | ||||
gi|15227028 | PTAC11 (DNA binding protein p24-related) | 69–84 | 2 | 0.7 | 3.02 | ||
At2g02740 | 81–89 | 2 | 0.7 | 2.91 | |||
96–103 | 2 | 0.0 | 2.58 | ||||
104–116 | 2 | 0.4 | 0.4 | 3.83 | 3.78 | ||
120–134 | 2 | 0.1 | −0.6 | 3.73 | 4.33 | ||
133–146 | 2 | 0.8 | 3.99 | ||||
196–204 | 2 | 0.1 | 0.1 | 3.34 | 3.26 | ||
205–218 | 2 | 0.3 | −0.6 | 3.23 | 5.77 | ||
219–252 | 3 | 0.6 | 3.76 | ||||
gi|15226791 | PTAC12 (unknown protein) | 106–121 | 2 | 0.6 | 2.66 | ||
At2g34640 | 127–142 | 2 | 0.6 | 4.41 | |||
190–206 | 2 | 0.1 | 3.03 | ||||
225–236 | 2 | 0.4 | 3.28 | ||||
250–259 | 2 | 0.4 | 3.28 | ||||
269–283 | 2 | −0.1 | 4.55 | ||||
280–294 | 2 | 0.3 | 4.79 | ||||
304–315 | 2 | −0.4 | 2.70 | ||||
329–351 | 3 | 0.2 | 4.88 | ||||
340–362 | 3 | 0.2 | 4.65 | ||||
gi|18398425At3g09210 | PTAC13 (KOW domain-containing transcription factor family protein) | 65–70 | 1 | −0.9 | 1.69 | ||
122–138 | 2 | −0.5 | 3.05 | ||||
174–185 | 2 | 0.6 | 2.51 | ||||
198–209 | 2 | −0.7 | 3.97 | ||||
285–298 | 2 | 0.1 | 4.11 | ||||
gi|42566980 | PTAC14 (SET domain-containing protein) | 118–131 | 2 | 0.8 | 3.29 | ||
At4g20130 | 224–230 | 1 | 0.6 | 1.70 | |||
250–260 | 2 | 0.1 | 2.92 | ||||
337–350 | 2 | −0.3 | 3.61 | ||||
357–383 | 3 | 0.5 | 5.15 | ||||
384–397 | 2 | 0.2 | 4.61 | ||||
398–415 | 2 | 1.0 | 2.72 | ||||
gi|15239573At5g54180 | PTAC15 (mitochondrial transcription termination factor-related) | 222–237 | 2 | −0.2 | 4.15 | ||
259–273 | 2 | 1.3 | 3.57 | ||||
347–361 | 2 | 0.7 | 3.70 | ||||
gi|42565672 | PTAC16 (expressed protein) | 120–130 | 2 | 0.2 | 3.10 | ||
At3g46780 | 149–164 | 2 | 0.3 | 3.13 | |||
237–248 | 2 | 0.8 | 2.87 | ||||
249–260 | 2 | −1.9 | −0.1 | 4.42 | 2.68 | ||
gi|15220146At1g80480 | PTAC17 (cobW domain-containing protein) | 89–102 | 2 | 0.4 | 2.66 | ||
208–218 | 2 | −1.7 | 3.10 | ||||
gi|15225202 | PTAC18 (expressed protein) | 75–93 | 2 | 0.7 | −1.1 | 4.00 | 4.64 |
At2g32180 | 116–130 | 2 | 0.5 | 5.68 |
Analysis of tandem MS (MS/MS) spectra is based on the Arabidopsis database (ftp://ftp.arabidopsis.org/home/tair/sequences/blast_datasets/).
National Center for Biotechnology Information/Arabidopsis Genome Initiative accession numbers.
Position of identified peptides within the database protein sequence.
The charge state of the measured ion (z).
The calculated deviation of the experimentally determined mass from the theoretical average mass of the peptide. Ara, Arabidopsis; Sin, S. alba.
xcorr value calculated using the sequest algorithm.