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. 2026 May 22;13:1808950. doi: 10.3389/fvets.2026.1808950

Table 4.

Spearman’s correlation analysis of ruminal fermentation products, blood biomarkers, and behavioral stress responses.

Variables Volatile fatty acids Ammonia Plasma hormones
Open-field behaviors Acetic acid Propionic acid Butyric acid Total VFA Acetic acid/ Rumen ammonia
Propionic acid Plasma ammonia Cortisol Aldosterone
Latency to enter −0.112 −0.200 −0.446† −0.208 0.275 −0.536* −0.695** −0.574* −0.320
Squares entered 0.213 0.125 0.161 0.116 −0.146 0.534* 0.777*** 0.266 0.313
Sniffing 0.344 0.413 0.404 0.342 −0.265 0.455† 0.569* 0.254 0.424†
Latency to bleat −0.113 −0.246 −0.417† −0.247 0.437† −0.276 −0.517* −0.642** 0.092
Bleats −0.036 0.164 0.185 0.063 −0.412 0.298 0.540* 0.483* 0.261
Plasma hormones
Cortisol −0.012 0.512* 0.485* 0.350 −0.561* 0.341 0.440†
Aldosterone 0.319 0.360 0.255 0.380 −0.140 0.394 0.404

Variables are pooled across treatments and periods (n = 17). *p < 0.05; **p < 0.01; ***p < 0.001; †0.05 ≤ p < 0.10.