Fig. 4.

The specificity of the anti-MBP antibodies toward recombinant substrates. Schematic description of the designed fusion proteins (A) Identification of the cleavage sites of Trx-MBP fusion proteins and nonmodified Trx generated by anti-MBP antibodies: SJL mice with EAE (white boxes), MS patient (red boxes), and C57BL/6 mice with EAE (blue boxes) (B). Data obtained by mass-spectrometry analysis and gel electrophoresis of degradation products (Fig. 8, which is published as supporting information on the PNAS web site).